Computed Tomography (CT) scan of the paranasal sinuses and microRNA expression in patients with Nasal polypi..

Ali, Mostafa Mahmoud Mohamed; Guindi, Sherif Safwat; Qotb, Mohamed; Ibrahim, Mohammad Mamdouh; Gaber, Sylvana N.; Abdelaleem, Omayma O.

doi:10.21608/fumj.2024.269412.1322

Computed Tomography (CT) scan of the paranasal sinuses and microRNA expression in patients with Nasal polypi..

Document Type : Full Length research Papers

Authors

¹ Otorhinolaryngology department, faculty of medicine, Fayoum university

² Otorhinolaryngology, ENT Department Faculty of Medicine, Fayoum University, Egypt

³ Professor of Otolaryngology Fayoum University

⁴ Department of Otorhinolaryngology-Faculty of Medicine - Fayoum University.

⁵ Department of Medical Microbiology and immunology - Fayoum university- Fayoum

⁶ Medical Biochemistry and Molecular Biology - Fayoum university- Fayoum

10.21608/fumj.2024.269412.1322

Abstract

Introduction: Nasal polypi is a prevalent clinical illness in the outpatient clinic in ENT. Nasal polyps are usually bilateral inflammatory lesions that arise from the ethmoid sinus and protrude into the nasal airway. MicroRNAs (miRNAs) are a class of non-coding RNAs. They are involved in controlling gene expression. MicroRNAs can control transcription and initiate translation under specific circumstances. Numerous variables influence the dynamic interaction between microRNAs and their target genes.
Aim of the study: to correlate between CT findings of the paranasal sinuses and microRNA 29 expression in patients with Nasal polypi.
Subjects and Methods: our research included one hundred participants—fifty of them had sinus polyps and the remaining fifty were normal. We took a thorough ENT history, including the patient's name, sex, age, chronic illnesses, and nasal symptoms. Complaints about smell and facial ache. All subjects had 3 ml of venous blood samples taken for RNA extraction and to find the expression of microRNA 29 using RT-PCR.
Results: Patients with complete opacification of the anterior ethmoids have a lower median (IQR) in terms of a high degree of underexpression of the miR-29 biomarker. However, no significant relationships with p-value >0.05 were found between the markers level and other CT PNS findings.
Conclusions: In this work, we found that individuals with nasal polyps significantly underexpressed microRNA 29, and that this underexpression was associated with many aspects of the patients' clinical presentation, including sense of nasal blockade, and full opacification of the anterior ethmoids.

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