ASSOCIATION OF UDP-GLUCORONYL TRANSFERASE 1-A7 POLYMORPHISM WITH HEPATOCELLULAR CARCINOMA AND LIVER CIRRHOSIS

Elnaggar, Haytham Abdelrahman; Ramzy, Iman Ismael; Mosaad, Nehad; Abd El Aal, Amal; El Baz, Heba

doi:10.21608/fumj.2024.262233.1305

ASSOCIATION OF UDP-GLUCORONYL TRANSFERASE 1-A7 POLYMORPHISM WITH HEPATOCELLULAR CARCINOMA AND LIVER CIRRHOSIS

Document Type : Full Length research Papers

Authors

¹ Department of chemical and clinical pathology, Faculty of medicine, Cairo University

² Department of Tropical Medicine,Faculty of Medicine, Cairo University

³ Professor of Chemical and Clinical Pathology,Faculty of Medicine-Cairo University

10.21608/fumj.2024.262233.1305

Abstract

Context: One of the most prevalent cancers worldwide is hepatocellular carcinoma (HCC). Enzymes called UDP-glucuronosyltransferases, or UGTs, are present in several living organisms, inclusive of bacteria and humans. Membrane-bound conjugating enzymes, or UGTs, catalyze the relocation of the glucuronic acid group of uridinediphosphoglucuronic acid (UDPGlcA) to the functional group of a specific substrate. This gene codes for many UDP-glucuronosyltransferases and is part of a multigene complex.
Aim: Determine whether hepatocellular cancer and liver cirrhosis are associated with the UDP-GLUCORONYL TRANSFERASE1-A7 polymorphism at codons 129 and 131.
Subjects and Methods: Seventy patients were enlisted from Cairo University's Kasr El Ainy tropical unit. To extract genomic DNA, whole blood was used as a sample. The real-time PCR method of melting curve analysis was used to identify the genetic variations in UGT1A7. examining codons 129 and 131 for mutations. Primer surrounding the polymorphisms intrigued in exon 1 of UGT1A7 and fluorescence fluorescence resonance energy transfer (FRET) probes were planned based on the detailed nucleotide arrangement (GenBank U39570).
Results: Compared to wild type, there was a high prevalence of heterozygous (1,2) and homozygous (2,2) codon 129,131 in HCC cases (1,1). Liver cirrhosis cases had higher frequencies of heterozygous and homozygous genotypes compared to the wild type, while controls had higher frequencies of the wild type genotype.
Conclusion: In conclusion, the polymorphism at codons 129 and 131 of UGT1A7 has been linked to hepatocellular carcinoma (HCC) and is therefore regarded as a risk factor for both liver cirrhosis and HCC.

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