ASSOCIATION OF UDP-GLUCORONYL TRANSFERASE 1-A7 POLYMORPHISM AT CODON 208 WITH HEPATOCELLULAR CARCINOMA AND LIVER CIRRHOSIS

Document Type : Full Length research Papers

Authors

1 Assistant lecturer of chemical and clinical pathology, Faculty of Medicine- Cairo University

2 Professor of Chemical and Clinical Pathology, Faculty of Medicine-Cairo University

3 Department of Tropical Medicine,Faculty of Medicine, Cairo University

4 Professor of Chemical and Clinical Pathology,Faculty of Medicine-Cairo University

Abstract

Context: Universally, hepatocellular carcinoma (HCC) is one of the foremost broad malignancies. UDP-glucuronosyltransferase (UGT) enzymes are found in a wide range of living things, including humans and microbes. Uridinediphosphoglucuronic acid's transfer (UDPGlcA) glucuronic acid group to a particular substrate's functional group is catalysed by membrane-bound conjugating enzymes called UGTs. The several UDP-glucuronosyltransferases that are encoded by this complicated locus include this gene.
Aim: Determine whether hepatocellular cancer and liver cirrhosis are associated with the UDP-GLUCORONYL TRANSFERASE1-A7 polymorphism at codon 208.
Subjects and methods: Seventy patients were drawn from the Kasr El Ainy tropical unit at Cairo University. A full blood sample was used to obtain genomic DNA. Melting curve analysis in real-time PCR was used to identify the genetic variations in UGT1A7. finding 208 codon mutations was done by screening. Fluorescence resonance energy transfer (FRET) probes and primers flanking the polymorphisms intrigued in UGT1A7's exon 1 were created using the issued nucleotide sequence (GenBank U39570).
Results: The findings demonstrated that HCC cases had higher levels of codon 208 (heterozygous (1,3) and homozygous (3,3) genotypes as compared to wild type (1,1). On the other hand, homozygous and heterozygous liver cirrhosis patients have the same frequency as wild type. When compared to heterozygous genotype, the frequency of wild genotype is higher in the control group.
Conclusion: Polymorphism at codon 208 in UGT1A7 has been linked to hepatocellular carcinoma (HCC) and is therefore regarded as a risk factor for both HCC and liver cirrhosis.

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Fayoum University Medical Journal
Volume 13, Issue 3
Medical Subspecialty
April 2024
Pages 72-81

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