Assessment of Micro RNAs 31 In Pathogenesis of Hypertrophic Scar

Abdel-Rahem, Talal; Shaker, Olfat; Mohamed, Mohamed Hassan; Rezk, Sara Hesham

doi:10.21608/fumj.2023.336859

Assessment of Micro RNAs 31 In Pathogenesis of Hypertrophic Scar

Document Type : Full Length research Papers

Authors

¹ Dermatology & STD and Andrology department, Fayoum University

² Biochemistry department, Cairo University

³ Dermatology, STDs and Andrology Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt.

⁴ Dermatology department, Faculty of Medicine, Fayoum University, Fayoum, Egypt

10.21608/fumj.2023.336859

Abstract

Introduction: A hypertrophic scar (HS) is a pathological result of wound healing. It is caused by the excessive accumulation of extracellular matrix components. MicroRNAs regulate protein-coding gene expression, participating in different biological processes. MiR-31 has a wide range of molecular targets and is specifically expressed in various tissues and organs.
Aim of the study: to evaluate the role of miR-31 in hypertrophic scar parthenogenesis.
Subjects and Methods: The study included 20 hypertrophic scar patients and 20 healthy subjects as a control. The level of the microRNAs in serum was estimated using serum isolation Then, cDNA was formed using the RT Kit. PCR will be conducted by real-time PCR for the amplification of the genes.
Results: There was a highly statistically significant difference between the two groups of miR-31. The level of mir-31 was much higher in the patient group than in the normal control.
Conclusion: Our findings suggest that miR-31 is pro-fibrotic and that its up-regulation stimulates fibrosis. This may provide a new direction for the study of the parthenogenesis of hypertrophic scars.

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